Msp1 RFLP in the human hexossaminidase β peptide (Hex B) gene

A frequent Pvu II RFLP of the human gastrin releasing peptide gene

SOURCE/DESCRIPTION: pB12, a 650 bp cDNA cloned at the Pst I and Hind III sites of pSP64 (Spindel et. al.). P0LYM0RPHIS: Pvu II detects two allelic fragments at 8.9 kb and 8.3 kb. OTHER COMMENTS: To obtain better separation of bands electrophorese on a 0.8% agarose gel until the xylene cyanol dye is at least 9 cm from the origin. ACKNOWLEDGEMENTS: We thank Ms. Denise Boorman for technical help.

MspI RFLP in the human fumarylacetoacetate hydrolase (FAH) gene.

A TaqI RFLP for the human arylsulfatase A gene.

A frequent Hindlll RFLP of the human fibrorrertin gene ( FNl )

SOURCE/DESCRIPTION: The probe used is a Hindlll/BamHI fragment of 1.4kb from the fibronectin recombinant, pFH6 (Kornblihtt, A.R. et al.). POLYMORPHISM: Hindlll (A/AGCTT) (New England, Bio-Labs, FRG) detects invariant bands at 4.75 and 1.7kb in addition to a simple two-allele polymorphism defined by the presence of a band of 7.1kb (F2) alternatively with two bands of 3.7 and 3.4kb (Fl) (Figure 1...

Natriuretic Peptide Type-B Gene

The structure of the mouse natriuretic peptide type-B (BNP) gene was determined by isolating and sequencing genomic clones. The mouse BNP gene was structurally similar to other natriuretic peptide genes and comprised three exons and two introns. Expression of the mouse BNP gene was found only in cardiac tissue as determined by ribonuclease protection analyses. Initiation of transcription was 31...